Protein Purification Reagents and Kits
Macherey-Nagel Protino Glutathione Agarose 4B, 10 mL
Protino Glutathione Agarose 4B (10 mL) Protino Glutathione Agarose 4B suspension for the purification of GST-tag proteins pack of 10 mL settled beaded agarose
STEMCELL Technologies RoboSep™ Human T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human T Cell Isolation Kit is designed to isolate T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This product can be used in place of the EasySep™ Human T Cell Enrichment Kit (Catalog #19051) for even faster cell isolations.
Gold Biotechnology Inc Nickel NTA Agarose Beads 100 mL
Nickel-NTA Agarose Resin has a binding capacity of ~50 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.
Dojindo Molecular Technologies Inc Dojindo ExoSparkler Exosome Protein Labeling Kit, Red, 1 to 10 μg Protein per Sample, 5 Samples
This kit allows for exosome protein fluorescent staining and enables observation of exosome dynamics without extracellular aggregation, covering steps from fluorescence labeling to purification. It can be used for staining purified exosomal membrane or protein, allowing imaging of labeled exosomes taken up by cells.
- Enables observation of exosome dynamics without extracellular aggregation.
- Includes filtration tubes for removal of unreacted dyes.
- Optimized protocol for exosome labeling.
- Recommended protein amount per sample: 1 to 10 μg.
- Recommended particle count per sample: 10 to 100 x 108 particles.
- Detection conditions (red): Ex 561 nm/Em 570 to 640 nm.
- Recommended purification methods include ultracentrifugation, immunoprecipitation, and magnetic beads.
- Store at 0°C to 5°C, protected from moisture.
STEMCELL Technologies EasySep™ Direct Human T Cell Isolation Kit, For processing: 100 mL whole blood processed
This cell separation kit can be used to isolate highly purified T cells directly from human whole blood by immunomagnetic negative selection.This kit targets non-T cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and can directly be used for functional assays, flow cytometry or DNA/RNA extraction.
STEMCELL Technologies EasySep™ Human Monocyte Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Monocyte Enrichment Kit is designed to isolate monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-monocyte cells and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to prevent nonspecific binding of monocytes. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which CD16+ cells are not removed, we recommend the EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion (Catalog #19058). For even faster cell isolations, we recommend the new EasySep™ Human Monocyte Isolation Kit (19359) (http://www.stemcell.com/en/Products/All-Products/EasySep-Human-Monocyte-Isolation-Kit.aspx) which isolates cells in as little as 12.5 minutes.
STEMCELL Technologies EasySep™ Human B Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human B Cell Isolation Kit is designed to isolate B cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched, desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. This product can be used in place of the EasySep™ Human B Cell Enrichment Kit (Catalog #19054) for even faster cell isolations.
STEMCELL Technologies EasySep™ Mouse Monocyte Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Monocyte Isolation Kit targets non-monocyte cells by labeling unwanted cells with antibodies and magnetic particles, and separates cells without columns using an EasySep™ magnet. Desired cells are simply poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture or cell-based assays.This product replaces the EasySep™ Mouse Monocyte Enrichment Kit (Catalog #19761) for even faster cell isolations.
Macherey-Nagel NucleoBond Xtra Maxi EF, 10 preps
NucleoBond Xtra Maxi EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A
STEMCELL Technologies EasySep™ Direct Human B Cell Isolation Kit, For processing: 100 mL whole blood processed
Isolate highly purified B cells directly from human whole blood without the need for density gradient centrifugation, RBC sedimentation or lysis. This platform provides fast and fully immunomagnetic negative selection.This cell isolation kit targets non-B cells for removal with EasySep™ Direct RapidSpheres™ and antibodies recognizing specific surface antigens. Unwanted cells are separated from desired cells using an EasySep™ magnet, and untouched target cells are then pipetted or poured into a new tube. Isolated cells can be used directly in downstream applications such as flow cytometry, culture or DNA/RNA extraction.